Document Type : Research Articles
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt.
Department, of Pathology, Faculty of Medicine, Zagazig University, Egypt.
Gynecology and Obstetrics department, Faculty of Medicine, Zagazig University, Egypt.
Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig University, Egypt.
Objective: To asses miR-379-5p expression in endometrial cancer (EC) and its correlation with ROR1 expression and to investigate the relation between miR-379-5p and ROR1 expressions and the clinicopathological picture of EC. Methods: Fifty female of EC were joined to this study. The gene expression of miR-379-5p (by quantitative real time-PCR) and ROR1 (by quantitative real time-PCR and immunohistochemistry) were studied in EC and normal nearby endometrial tissue. Results: The gene expression of miR-379-5p was significantly downregulated while that of ROR1 was significantly upregulated in EC tissues compared to adjacent normal endometrial tissues. Furthermore, miR-379 and ROR1 expressions significantly associated with tumor stage (P< 0.045), grade (P< 0.001), myometrial invasion (P <0.001) and LN metastasis (P< 0.034). In addition, miR-3795p and ROR1 gene expression were negatively correlated (r = -0.746, P < 0.001). Conclusions: In EC, miR-379-5p can be used as a diagnostic marker, and ROR1 could be a potential target of miR-379-5p.