ADAM10 Gene Polymorphism and Its Relationship to Hepatocellular Carcinoma in Egyptian HCV Patients Receiving Direct-Acting Antiviral Therapies (DAAs)

Document Type : Research Articles

Authors

1 Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Egypt.

2 Department of Epidemiology and Preventive Medicine, National Liver Institute, Menoufia University, Egypt.

3 Department of Clinical Pathology, National Liver Institute, Menoufia University, Egypt.

4 Department of Clinical Microbiology and Immunology Department, National Liver Institute, Menoufia University, Egypt.

5 Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Egypt.

6 Department of Clinical Pharmacy, Pharmacy Collage, Menoufia University, Egypt.

Abstract

Objective: The aim of this study was to investigate the ADAM 10 rs.653765 SNP genetic polymorphism in the hepatocellular carcinoma occurrence (de novo and post DAAs). Methods: This study was conducted on 360 participants divided to 4 groups. Group 1: 90 chronic adult patients infected with HCV received DAAs regimens and evolved HCC during the period of follow up. Group 2: Another 90 HCV patients received the same DAAs regimens and did not show HCC manifestations during the same follow up period. Group 3 included 90 de novo HCC patients (did not receive any DAAs). Finally, 90 apparently healthy participants as group 4. Clinical and laboratory data were evaluated, and ADAM 10 genotyping were performed using qPCR. Results: The study showed statistically significant between HCC de novo and HCC deterioration on top of DAAs according to three scoring systems (Child Pugh, BCLC and HKLC) with p- value <0.05. Regarding ADAM10 gene polymorphism, the study showed a significant difference between CC versus CT+TT genotypes of HCC groups according to Child Bugh, BCLC and HKLC staging systems. Yet, no significant difference was found when ADAM10 genotypes and allele frequencies were compared between the four different studied groups. No difference in the survival rate between HCC de novo and on the top of DAAs but more aggressive stages with HCC on top of DAAs. Conclusion: ADAM10 genotypes did not show any significant association with HCC. Also, no differences in the death rate recorded between the de novo HCC and HCC post DAAs treatment with statistical significant worse staging of HCC post DAAs and were noted. the study showed a significant difference between CC versus CT+TT genotypes of HCC groups according to Child Bugh, BCLC and HKLC staging systems.
 

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Asian Pacific Journal of Cancer Prevention
Volume 24, Issue 1
January 2023
Pages 149-155

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History

  • Receive Date: 24 May 2022
  • Revise Date: 02 August 2022
  • Accept Date: 17 January 2023

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