Protective Effect of Adenosine Triphosphate against 5-Fluorouracil-Induced Oxidative Ovarian Damage in vivo

Ozer, Mehmet; Ince, Sefa; Altuner, Durdu; Suleyman, Zeynep; Cicek, Betul; Gulaboglu, Mine; Mokhtare, Behzad; Gursul, Cebrail; Suleyman, Halis

doi:10.31557/APJCP.2023.24.3.1007

Protective Effect of Adenosine Triphosphate against 5-Fluorouracil-Induced Oxidative Ovarian Damage in vivo

Document Type : Research Articles

Authors

¹ Department of Obstetrics and Gynecology, Izmir Tepecik Regional Training Research Hospital, Izmir, Turkey.

² Department of Obstetrics and Gynecology, Regional Training Research Hospital, Erzurum, Turkey.

³ Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey.

⁴ Department of Physiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey.

⁵ Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.

⁶ Department of Pathology, Faculty of Veterinary, Ataturk University, Erzurum, Turkey.

10.31557/APJCP.2023.24.3.1007

Abstract

Objective: The aim of our study was to analyze the effect of ATP on possible ovarian damage of 5-FU in rats. Methods: The animals were divided to three groups; healthy group (HG), 5-FU alone group (FUG) and ATP+5-FU administered group (AFU). The ATP 4 mg/kg was injected intraperitoneally (IP) into the AFU group. The same volume of saline (0.9% NaCl) as the solvent was administered intraperitoneally to the HG and FUG groups. One hour after administering ATP and solvent, 5-FU 100 mg/kg was injected intraperitoneally to the animals in the AFU and FUG groups. ATP was administered to the animals once a day for 10 days. On the 1st, 3rd and 5th days of 5-FU, one dose (total of 3 doses) was administered. On day 10, the animals were euthanasia with high-dose anaesthesia and ovarian tissues were removed. The removed ovaries were analyzed biochemically andhistopathological. Result: ATP significantly suppressed both the increase in MDA and IL-6 levels, and the decrease in tGSH, SOD and CAT levels. Treatment with ATP significantly suppressed the severe vacuolization and primordial follicle degeneration induced by 5-FU in our study. Conclusion: ATP was possible to be useful for the treatment of 5-FU-induced ovarian damage.

Keywords