Evaluation of the Anti-Carcinogenic Effect of Centella Asiatica on Oral Cancer Cell Line: In vitro Study

Document Type : Research Articles

Authors

1 Pg trainee, India.

2 Department of Oral and Maxillofacial Pathology, SRM Dental College, SRM University, Ramapuram, Tamilnadu, India.

3 Department of Oral Pathology SRM Dental College SRM University Chennai, India.

4 Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, Chennai, India.

Abstract

Objective: To evaluate the anti-carcinogenic effect of Centella Asiatica on to evaluate the  Anti-Carcinogenic Effect of Centella Asiatica on Oral Cancer Cell Line oral cancer cell line. Materials and methods:  Oral Cancer cell line and normal oral keratinocyte cell line were procured.Centella asiatica extract was prepared. The cells were then subjected to the test herbal specimens -Centella asiatica extract in succeeding concentrations of 25 µg/ml, 50 µg/ml, 100 µg/ml at time intervals of 24,48 and 72 hours. Cisplatin (2 µg/ml, 4 µg/ml, 6 µg/ml, 8 µg/ml) was used as a positive control. This experiment was done in triplets. Results: The study revealed that the p values were less than 0.05 at concentration 12.5µg/ml, 25µg/ml, 50 µg/ml,100 µg/ml and time period of 24hrs,48hrs,72hrs, thus implying that at these concentrations and time period, the obtained data were statistically significant, thus indicating that there is a statistically significantly decreases in the viable cells as the concentration of the drug as a time period increases The results reveals that  centella asiatica possess potential effect of anti-carcinogenic, effect when compared to positive control (Cisplatin). Conclusion: The current study reveals that Centella asiatica has an potential anti-carcinogenic effect on oral cancer cell line. So this can be used to treat oral cancer with minimal crippling as compared with allopathic drugs.

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Volume 24, Issue 5
May 2023
Pages 1695-1700
  • Receive Date: 04 January 2023
  • Revise Date: 23 March 2023
  • Accept Date: 17 May 2023
  • First Publish Date: 17 May 2023