The Association of pre-miR27a Gene Polymorphism and Clinicopathological Data in Thai Breast Cancer Patients

Document Type : Research Articles


1 Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

2 Division of Research and Academic Support, National Cancer Institute, Thailand.

3 Faculty of Medical Technology, Rangsit University, Prathum Thani Thailand.

4 Pathobiology Unit, Department of Biomedical Science, Faculty of Science, Rangsit University, Prathum Thani Thailand.


Background: MiR27a plays an important role in carcinogenesis, cell proliferation, apoptosis, invasion, migration and angiogenesis. Several studies have identified an important role of pre-miR27a (rs895819) A>G polymorphism in several types of cancer. This research aims to investigate the association of pre-miR27a (rs895819) A>G and breast cancer susceptibility, clinicopathological data and survival. Blood DNA samples of 143 Thai breast cancer patients and 100 healthy Thai women were studied for pre-miR27a (rs895819) A>G polymorphism using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Results: The results revealed that the frequency of pre-miR27a (rs895819) A>G genotypes was not statistically significant different between breast cancer patient and normal control subjects. The rs895819 A>G genotype was significantly associated with clinicopathological parameter of grade III differentiation (P = 0.006), progesterone receptor (P = 0.011) and triple negative (P = 0.031) in breast cancer patients, but not with breast cancer susceptibility. Conclusion: The pre-miR27a (rs895819) A>G genotype was significantly associated with poorly differentiated, progesterone receptor and triple-negative in breast cancer patients. Therefore, pre-miR27a (rs895819) A>G may be used as a biomarker for poor prognosis.


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