The Selective Cytotoxicity of Quercus Brantii Lindl. Galls on A375 and SK-MEL-3 Human Malignant Melanoma Cell Lines

Yosefsani, Bahareh Sadat; Mohajer, Kamyar; Qobadi, Ali; Aghazadeh, Elahe; Shirani, Kobra; Pourahmad, Jalal

doi:10.31557/APJCP.2023.24.7.2383

The Selective Cytotoxicity of Quercus Brantii Lindl. Galls on A375 and SK-MEL-3 Human Malignant Melanoma Cell Lines

Document Type : Research Articles

Authors

¹ Department of Traditional Pharmacy, School of Persian Medicine, Iran University of Medical Sciences, Tehran, Iran.

² Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

³ Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

10.31557/APJCP.2023.24.7.2383

Abstract

This study aimed to find out the mechanism of cytotoxic effects of galls of Quercus Brantii on A375 and SK-MEL-3 melanoma and AGO-1522 normal human fibroblast cell lines for the first time. Therefore, cell viability and cytotoxic activities were evaluated. Furthermore, ROS formation, lipid peroxidation, and release of cytochrome-c were also assessed. The results revealed that the extract of these galls at a concentration of 0.05 mg/ml significantly (P<0.001) increased cytotoxicity, ROS formation, TBARS formation, and cytochrome-c release in A375 and SK-MEL-3 melanoma cell lines compared to AGO-1522 normal human fibroblast. These results demonstrated that these galls can be considered a promising candidate which acts in synergy with anticancer agents used in the clinical treatment of human malignant melanoma.

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