GLUT5, GLUT7, and GLUT11 expression and Bcl-2/Bax ratio on Breast Cancer Cell Line MCF-7 Treated with Fructose and Glucose

Margawati, Harlindah; Yustisia, Ika; Hardjo, Marhaen; Natsir, Rosdiana; Aziz, Ilhamuddin; Hafiyani, Lia; Aswad, Hijral

doi:10.31557/APJCP.2023.24.11.3917

GLUT5, GLUT7, and GLUT11 expression and Bcl-2/Bax ratio on Breast Cancer Cell Line MCF-7 Treated with Fructose and Glucose

Document Type : Research Articles

Authors

¹ Master Programme of Biomedical Sciences, Graduate School Universitas Hasanuddin, Makassar, Indonesia.

² Makassar Medical State Laboratory, Indonesian Ministry of Health, Makassar, Indonesia.

³ Department of Biochemistry, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia.

⁴ Department of Pharmacology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia.

⁵ Hasanuddin University Medical Research Centre (HUMRC), Makassar, Indonesia.

10.31557/APJCP.2023.24.11.3917

Abstract

Objective: Fructose and glucose are types of sugars commonly found in the diet that have been linked to cancer development. Glucose transporters (GLUTs) are facilitating the uptake of these hexoses. Expression of GLUT5 is higher in cancer cells than in healthy tissue. GLUT7 and GLUT11 facilitate the transport of glucose and fructose; however, their expression in breast cancer has not been extensively studied. The Bcl-2 family has been known as a regulator of the cell’s survival and death. Here, we investigated the effect of the fructose-glucose combination in MCF-7 breast cancer cells on the viability, migration, and expression of GLUT5, GLUT7, GLUT11, and Bcl-2/Bax ratio. Methods: Breast cancer cells MCF-7 were treated with fructose, glucose, and combinations of fructose:glucose (75%:25%, 50%:50%, 25%:75%). Cell viability was assessed using an MTT test. Cell migration was examined with a wound-healing assay. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the mRNA expression of GLUT5, GLUT7, GLUT11, and Bcl-2/Bax. Results: The viability and migration of MCF-7 breast cancer cells elevated when treated with a combination of fructose and glucose, and glucose alone, compared to fructose alone. The expression levels of GLUT5 and GLUT7 were highest in combination of fructose:glucose (75%:25%). Conversely, the expression of GLUT11 was consistently low across all treated media. The highest Bcl-2/Bax ratio was shown in fructose:glucose combination (25%:75%). Conclusion: The viability, migration, and Bcl-2/Bax ratio are enhanced in the combination media with higher glucose. In contrast, when the fructose composition was higher in the media, expression of GLUT5 and GLUT7 increased.

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