Effect of Laetrile Vinblastine Combination on the Proliferation of the Hela Cancer Cell Line

Yasin, Youssef Shakuri; Jumaa, Azal Hamoody; Jabbar, Shilan; Abdulkareem, Ahmed Hazem

doi:10.31557/APJCP.2023.24.12.4329

Effect of Laetrile Vinblastine Combination on the Proliferation of the Hela Cancer Cell Line

Document Type : Research Articles

Authors

¹ Bilad Alrafidain University College, Diyala, 32001, Iraq.

² Iraqi National Cancer Research Center/ University of Baghdad, Baghdad, Iraq.

³ Department of Biology ,College of Science, University of Kirkuk, Kirkuk, Iraq.

10.31557/APJCP.2023.24.12.4329

Abstract

Aim: This study aimed to evaluate the inhibitory effect of laetrile, vinblastine, and their mixture on cervical cancer cells and probe potential synergistic consequences. Method: The study scrutinized the inhibitory impact of laetrile vinblastine and their mixture on the growth of human cervical cancer cells (Hela cancer cell line). The cells were incubated for 24, 48, and 72 hours with concentrations varying from 1 microgram to 10,000 micrograms of each substance. Result: study results showed, the combination of vinblastine and laetrile effectively reduced the viability of human cervical cancer cells. This effect was stronger than the individual cytotoxic effects of each compound. The results suggest that the cytotoxicity of the vinblastine and laetrile combination increases with higher concentrations of the compounds. Additionally, the study revealed a synergistic effect between the mixture ingredients, particularly at the lowest and highest concentrations during the 24 and 72-hour incubation periods. Conclusion: The antiproliferative effect of (the combination of laetrile and vinblastine) was greater than the antiproliferative effect of either compound used alone, suggesting a synergistic relationship between the two.

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