M2 Macrophage Prominently Distributed in the Rat’s Colon of DMH-Induced Inflammation Associated Colorectal Cancer

Omadhika, Windy Arensya; Solikhah, Solikhah; Adrianto, Albertus Ari; Purwestri, Yekti Asih; Paramita, Dewi Kartikawati

doi:10.31557/APJCP.2024.25.4.1357

M2 Macrophage Prominently Distributed in the Rat’s Colon of DMH-Induced Inflammation Associated Colorectal Cancer

Document Type : Research Articles

Authors

¹ Faculty of Biology Universitas Gadjah Mada, Yogyakarta, Indonesia.

² Digestive Surgery Department of Medical Faculty Universitas Diponegoro, Semarang, Indonesia.

³ Laboratory of Biochemistry, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.

⁴ Study Center for Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia.

⁵ Department of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

⁶ Integrated Research Laboratory, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

10.31557/APJCP.2024.25.4.1357

Abstract

Objective: The aim of this study is to examine the M1 and M2 macrophages distribution in the rat’s colon of DMH-induced inflammation associated colorectal cancer. Methods: Colon tissue of three groups of 4 rats that induced using 1,2 dimethylhydrazine (DMH) at 30 mg/kg bw every week for 9, 11, and 13 weeks were used. The M1 and M2 distribution was examined by using antibody anti iNOS for M1 and anti-CD163 for M2 with immunohistochemistry method. The data was presents in figure and table in the form of percentage. Result: M1 macrophage was found in all groups in the low distribution level (25% - 50%), while M2 macrophage was observed in all groups with 100% distribution. In the longer period of DMH induction, M2 macrophages was distributed more abundant. Conclusion: All of the rat’s colon showing chronic inflammation that led to the tumorigenesis.

Keywords