The Cytotoxic Effect of Ciprofloxacin Laetrile Combination on Esophageal Cancer Cell Line

Jumaa, Azal Hamoody; Abdulkareem, Ahmed Hazem; Yasin, Youssef Shakuri

doi:10.31557/APJCP.2024.25.4.1433

The Cytotoxic Effect of Ciprofloxacin Laetrile Combination on Esophageal Cancer Cell Line

Document Type : Research Articles

Authors

¹ Iraqi National Cancer Research Center, University of Baghdad, Baghdad, Iraq.

² Bilad Alrafidain University College, Department of Pharmacy, Diyala, Iraq.

³ Bilad Alrafidain University College, Anesthesia Department, Diyala, Iraq.

10.31557/APJCP.2024.25.4.1433

Abstract

Objective: aim of this study was to examine the synergistic effect between the antibacterial drug ciprofloxacin and the natural compound laetrile on esophageal cancer cells, specifically focusing on their combined cytotoxic effect. Methods: The combined cytotoxic effects of two alternative incubation durations (24 and 72 hours) were studied using an esophageal cancer cell line. Ciprofloxacin, laetrile, and their combinations were tested at concentrations ranging from 1 to 1000 micrograms/milliliter, to enhance the safety of the combination, the concentrations of the combination constituents were reduced by half compared to when they are used individually, the combination index was then calculated to estimate the components’ possible synergistic effects. Result: The results indicate that the combined cytotoxicity of ciprofloxacin and laetrile was greater than the cytotoxicity of either ciprofloxacin or laetrile alone, the combination cytotoxicity increased with higher concentrations and longer incubation periods, in other words, the cytotoxicity pattern of the combination was time-dependent (cell-cycle specific), and concentration dependent, (cell-cycle non-specific). Conclusion: The study found that the combination of ciprofloxacin and laetrile had a greater inhibitory effect on the growth of esophageal cancer cells compared to ciprofloxacin or laetrile alone. This suggests a synergistic effect between the components of the mixture, which can be attributed to a complementary mechanism between the ingredients in the combination.

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