Blood-Based FTIR Spectroscopy as a Minimally Invasive Diagnostic Method for Colorectal Cancer: A Meta-Analysis

Document Type : Systematic Review and Meta-analysis


1 Department of Biological Sciences, College of Science, University of Santo Tomas, Manila, Philippines.

2 The Graduate School, University of Santo Tomas, Manila, Philippines.

3 Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.

4 Department of Medical Technology, College of Allied Medical Professions, Angeles University Foundation, Angeles City, Philippines.


Objective: This meta-analysis aims to pool the results of existing studies to obtain more precise estimates on the diagnostic efficiency of the Fourier transform infrared (FTIR) spectroscopy in detecting CRC using blood-based samples. Methods: A comprehensive database search identified 4,931 studies that were screened for eligibility. Relevant data were then extracted and collated and analyzed using Meta-DiSc 1.4 to measure the pooled diagnostic accuracy, sensitivity, specificity, likelihood ratio, and diagnostic odds ratio and presented in forest plots. Results: The pooled sensitivity across all six data entries was 86.10% (p = 0.20), and the specificity was 91.2% (p < 0.001). The pooled positive likelihood ratio was 9.84 (p < 0.001), indicating a strongly moderate diagnostic value, while the negative likelihood ratio was 0.16 (0.12), suggesting moderately decreased efficacy of FTIR spectroscopy in ruling out the disease. The pooled AUC was found to be at 0.94 which indicate excellent discriminating potential of FTIR of the method. Conclusion: Overall, the study suggests that FTIR spectroscopy has potential as minimally invasive diagnostic method for CRC using plasma samples.


Main Subjects