Investigation of TLR2 (-196 to -174del) and TLR9 (T-1486C) Gene Polymorphisms Association with Inflammatory Bowel Diseases

Document Type : Research Articles


1 Department of Biotechnology, Institute of Natural and Applied Sciences, University of Niğde Ömer Halisdemir, Niğde, Turkey.

2 Biotechnology- Proteomics and Molecular Biology, Africa City of Technology, Ministry of Higher Education And Scientific Research, Khartoum, Sudan.

3 Department of Mathematics and Sciences Education, Faculty of Education, University of Kocaeli, Kocaeli, Turkey.

4 Department of Biotechnology, Faculty of Science and Letters, University of Niğde Ömer Halisdemir, Niğde, Turkey.


Background: Inflammatory bowel diseases (IBD), Crohn’s disease (CD), and ulcerative colitis (UC) are diseases that result from the combined effects of a predisposing genetic background and several environmental factors, including smoking. Some genes can influence these diseases through genetic inheritance, and their regulation is explained by gene polymorphism. However, Toll-like receptor (TLR) genes have been identified as susceptibility genes for CD and UC. Methods: A case-control study was performed on a Turkish population composed of 105 healthy controls and  79 CD, 77 UC patients genotyped by Allele-specific PCR and PCR–RFLP for TLR9 (T-1486C) and TLR 2 (-196 to -174del) gene. Genotype and allele frequencies of TLR9 (T-1486C) and TLR 2 (-196 to -174del) gene polymorphisms compared to allele frequencies in CD and UC patients. Results: No statistically significant findings were found between the CD, UC patients, and the control group in terms of both genotype distributions and allele frequencies for TLR 9 (T-1486C; rs187084) and TLR 2 (-196 to -174del; rs111200466) gene polymorphisms in a Turkish population (P > 0.05). Conclusion: No association was found between the TLR2 (rs111200466) and TLR 9 (rs187084) gene polymorphisms among IBD patients and the control groups in the Turkish population.


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