Cytotoxic Effect of YH239-EE and Its Enantiomer on MCF7 Cell Line

Document Type : Research Articles


1 Department of Pharmacology, College of Medicine University of Baghdad, Baghdad, Iraq.

2 Dr. Hany Akeel institute, Iraqi Medical research center, Baghdad, Iraq.

3 Bilad Alrafidain University College, Baqubah, Iraq.


Objective: This study aimed to discover the cytotoxic effect of YH239-EE and YH239 alone and their enantiomer potency in cytotoxic effect on the MCF7 cell line. Methods: We used the cytotoxic study on MDM2 cell lines by detecting the percentage of apoptosis and necrosis by annexin v methods. Result: This result shows that YH239-EE causes more apoptosis and necrosis 40% in comparison to YH239 without ethyl ester, about 4.92 %,  and The (+) enantiomer of YH239-EE demonstrated a markedly higher induction of apoptosis and necrosis (84.48%) in MCF7 cells compared to the (-) enantiomer (48.71%). Conclusion: The ethyl ester group in YH239-EE might play a crucial role in enhancing the compound’s ability to induce cell death, and The high efficacy of the (+) enantiomer of YH239-EE in inducing cell death in MCF7 cells suggests it may be a more promising therapeutic candidate for breast cancer treatment, specifically for subtypes represented by MCF7 cells.


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