The Association of pri-miR34 b/c Gene Polymorphism and Clinicopathologic Data in Breast Cancer Patients

Kongton, Thaworn; Sanguansin, Sirima; Saelee, Pensri; Thongmee, Acharawan; Pongstaporn, Wanida

doi:10.31557/APJCP.2024.25.7.2415

The Association of pri-miR34 b/c Gene Polymorphism and Clinicopathologic Data in Breast Cancer Patients

Document Type : Research Articles

Authors

¹ Biomedical Science Graduate Program, Department of Medical Science, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand

² Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

³ Devision of Research and Academic Support, National Cancer Institute Thailand.

⁴ Department of Medical Science, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand.

10.31557/APJCP.2024.25.7.2415

Abstract

Background: MiR-34b/c takes an important role in various aspects of carcinogenesis. Notably, pri miR34b/c (rs4938723) T>C polymorphism has been identified as a significant biomarker in various kinds of cancer. The objective of this study was to explore whether pri-miR34b/c rs4938723) T>C was associated with breast cancer susceptibility. Moreover, the association of pri-miR34b/c (rs4938723) T>C and clinicopathologic data, including survival outcomes, were studied in Thai breast cancer patients. Methods: DNA extracted from the blood of 100 Thai female breast cancer patients and 100 Thai healthy women were investigated for pri-miR34b/c (rs4938723) T>C polymorphism using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Results: There was no statistically significant difference between the frequency of pri miR34b/c (rs4938723) T>C genotype between Thai breast cancer patients and normal subjects. This study showed that there is no association between pri-miR34b/c (rs4938723) genotypes and breast cancer susceptibility, clinicopathologic parameters, and survival time. However, age greater than 50 and histologic grade III were the prognostic factors affecting survival in breast cancer patients (p=0.017, p=0.010, respectively). Conclusion: The pri-miR34b/c (rs4938723) genotypes had no association with cancer susceptibility and clinicopathologic parameters in Thai breast cancer patients. Patients with older age and patients with higher histologic grade, but not the pri miR34b/c (rs4938723) genotype, affected survival time among breast cancer patients.

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