Refinement of Risk-Stratification of Cytogenetically Normal Acute Myeloid Leukemia Adult Patients by MN1 Expression

Ningombam, Aparna; Verma, Deepak; Kumar, Rajive; Singh, Jay; Ali, M Shadab; Pandey, Avanish Kumar; Singh, Inder; Bakhshi, Sameer; Sharma, Atul; Pushpam, Deepam; Palanichamy, Jayanth Kumar; Tanwar, Pranay; Ranjan Singh, Amar; Chopra, Anita

doi:10.31557/APJCP.2024.25.7.2283

Refinement of Risk-Stratification of Cytogenetically Normal Acute Myeloid Leukemia Adult Patients by MN1 Expression

Document Type : Research Articles

Authors

¹ Laboratory Medicine, JPNATC, AIIMS, New Delhi, India.

² Laboratory Oncology, Dr. BRAICH, AIIMS, New Delhi, India.

³ Pulmonary Medicine, AIIMS, New Delhi, India.

⁴ Neurology, AIIMS, New Delhi, India.

⁵ Medical Oncology, AIIMS, New Delhi, India.

10.31557/APJCP.2024.25.7.2283

Abstract

Introduction: Acute myeloid leukemia with normal cytogenetics (CN-AML) represents a heterogeneous group having diverse genetic mutations. Understanding the significance of each of these mutations is necessary. In this study, we evaluated the prognostic role of MN1 expression in adult CN-AML patients. Method: One hundred and sixty-three de-novo adult AML patients were evaluated for MN1 expression by real-time PCR. MN1 expression was correlated with the clinical characteristics of the patients and their outcomes. Results: Higher MN1 expression was associated with NPM1 wild-type (p<0.0001), CD34 positivity (p=0.006), and lower clinical remission rate (p=0.027). FLT3-ITD and CEBPA mutations had no association with MN1 expression. On survival analysis, a high MN1 expression was associated with poor event-free survival (Hazard Ratio 2.47, 95% Confidence Interval: 1.42-4.3; p<0.0001) and overall survival (Hazard Ratio 4.18, 95% Confidence Interval: 2.17-8.08; p<0.0001). On multivariate analysis, the MN1 copy number emerged as an independent predictor of EFS (p<0.0001) and OS (p<0.0001). Conclusion: MN1 expression is an independent predictor of outcome in CN-AML.

Keywords