Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA

Document Type : Research Articles

Authors

1 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

2 Craniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

3 Department of Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.

4 Department of Pulmonary and Critical Care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

5 Department of Oral and Maxillofacial Surgery, Sina Hospital, Tehran University Of Medical Sciences, Tehran, Iran.

6 Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

7 Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

8 Hepato-Pancreato-Biliary and Transplant Surgery, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Siences, Tehran, Iran.

9 Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran.

10 Health Management, Policy Making and Economic Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

11 Health Information Management Research Center, Tehran University of Medical Sciences, Tehran, Iran.

12 School of Advanced Technologies in Medicine, Department of Biotechnology, Shahid Bheshti University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis. Methods: In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique. Results: Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people. Conclusion: In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended.

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