Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA

Bahrami, Naghmeh; Pirrafiee, Maryam; Azadi, Fatemeh; Azimnejad, Rezvan; Fotook Kiaei, Seyedeh Zahra; Abbasi, Amir Jalal; Kazempour Dizaji, Mehdi; Lookzadeh, Somaieh; Nejatollahi, Seyed Mohammad Reza; Daustani, Mahya; Dargahi, Hossein; Mohamadnia, Abdolreza

doi:10.31557/APJCP.2024.25.7.2265

Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA

Document Type : Research Articles

Authors

¹ Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

² Craniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

³ Department of Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.

⁴ Department of Pulmonary and Critical Care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

⁵ Department of Oral and Maxillofacial Surgery, Sina Hospital, Tehran University Of Medical Sciences, Tehran, Iran.

⁶ Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

⁷ Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

⁸ Hepato-Pancreato-Biliary and Transplant Surgery, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Siences, Tehran, Iran.

⁹ Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran.

¹⁰ Health Management, Policy Making and Economic Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

¹¹ Health Information Management Research Center, Tehran University of Medical Sciences, Tehran, Iran.

¹² School of Advanced Technologies in Medicine, Department of Biotechnology, Shahid Bheshti University of Medical Sciences, Tehran, Iran.

10.31557/APJCP.2024.25.7.2265

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis. Methods: In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique. Results: Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people. Conclusion: In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended.

Keywords