Document Type : Research Articles
Authors
1
Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, MAHSA University, Selangor, Malaysia.
2
School of Dentistry, Management and Science University, Shah Alam, Selangor, Malaysia.
3
Animal Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
4
Health Research Centre, Jazan University, Jazan, Saudi Arabia.
5
Department of Microbiology, Faculty of Medicine, Manipal University College, Jalan Padang Jambu, Bukit Baru, Melaka, Malaysia.
6
Pharmacology Unit, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Subang Jaya, Selangor, Malaysia.
7
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Malaysia.
Abstract
Objective: Leukemia is a group of hematologic malignancies in the bonemarrow that arise from the dysfunctional proliferation of developing leukocytes. It is classified as either acute or chronic based on the rapidity of proliferation and as myelocytic or lymphocytic based on the cell of origin. Newcastle Disease Virus (NDV) is an avian paramyxovirus, which has been demonstrated to possess significant oncolytic activity against mammalian cancers because its ability to kill tumor cells with limited toxicity to normal cells. Methods: In this study, the morphophical changes and apoptosis induction of WEHI 3B leukemia cell line treated with NDV strain AF2240 were studied by scanning electron microscopes and transmission electron microscopes techniques. Result: Electron microscopy indicated that NDV strain AF 2240 significantly altered cell morphology and reduced cell viability. Furthermore, early apoptosis was observed 6 h post-inoculation by fluorescence microscope. Conclusion: Our results suggest that NDV has ability to induce significant apoptoic structural changes in WEHI 3B leukemia cell line. These findings provide new insights into the mechanism of action of NDV virotherapy and could lead to the development of more effective treatments for leukemia.
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