Oncolytic Newcastle Disease Virus and Photodynamic Therapy as Dual Approach for Breast Cancer Treatment

Al-Shammari, Shaymaa Majeed Hamzah; Al-Khafaji, Ahmed S. K.; Hassan, Haider A.; Salman, Marwa Ibrahim; Al-Shammari, Ahmed Majeed

doi:10.31557/APJCP.2024.25.9.3111

Oncolytic Newcastle Disease Virus and Photodynamic Therapy as Dual Approach for Breast Cancer Treatment

Document Type : Research Articles

Authors

¹ Ministry of Health, Al Diwaniya Health Bureau, Women and Children Hospital, Baghdad, Iraq.

² Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.

³ Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.

⁴ Department of Experimental Therapy, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad, Iraq.

10.31557/APJCP.2024.25.9.3111

Abstract

Objective: We hypothesized that attacking cancer cells by combining various modes of action can hinder them from taking the chance to evolve resistance to treatment. Incorporation of photodynamic therapy (PDT) with oncolytic virotherapy might be a promising dual approach to cancer treatment. Methods: NDV AMHA1 strain as virotherapy in integration with aminolaevulinic acid (ALA) using low power He-Ne laser as PDT in the existing work was examined against breast cancer cells derived from Iraqi cancer patients named (AMJ13). This combination was evaluated using Chou–Talalay analysis. Results: The results showed an increased killing rate when using both 0.01 and 0.1 Multiplicity of infection (MOI) of the virus when combined with a dose of 6172.8 photons/gm (ph/gm) of PDT focused on cancer cells. Conclusion: integration of the attenuated NDV–AMHA1 strain with photodynamic therapy has a synergistic killing effect on breast cancer cells in vitro, suggesting that this strategy could have clinical application to overcome breast cancer.

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