Quantification of the Most Effective X-Ray Dosage for Inhibiting Invasion of Glioma Cell Line

Ghasemi, Elham; Zamanzade, Zeynab; Seif, Fatemeh; Mondanizadeh, Mahdieh

doi:10.31557/APJCP.2024.25.12.4299

Quantification of the Most Effective X-Ray Dosage for Inhibiting Invasion of Glioma Cell Line

Document Type : Research Articles

Authors

¹ Student Research Committee, Arak University of Medical Sciences, Arak, Iran.

² School of Allied Medical Sciences, Department of Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

³ Department of Biotechnology and Molecular Medicine, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

10.31557/APJCP.2024.25.12.4299

Abstract

Background: One key feature that distinguishes cancerous cell populations from their normal counterparts is a heightened tendency towards uncontrolled growth and invasive activity. Therapeutic techniques like radiotherapy can impact the viability and invasive behavior of cancer cells by modifying the structure of DNA and inducing programmed cell death. Methods: This research is an experimental study and involved a comprehensive investigation into the effects of 6MV X-ray radiotherapy on various absorbed doses (ranging from 4 to 10 Gy by steps 2Gy) on toxicity, migration, and colony formation in C6 glioblastoma cellular cultures. Results: Our detailed analysis revealed that the cytotoxic responses increased in a dose-dependent manner, while there was a significant decrease in both the ability to migrate and form colonies in the C6 cell line. Conclusions: This thorough examination provides new insights into the way malignant cell populations respond to ionizing radiation in a dose-dependent manner within a clinical setting. By directly impacting cellular functions and causing disturbances, this occurrence leads to a slowing down of disease progression.

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