Objectives: To study the predictive role of tumor-associated neutrophils in early luminal HER2-negative breast cancer. Materials and Methods: This is a retrospective study conducted on 60 women cases aged from 31 to 79 years underwent surgery for luminal HER2-negative ductal breast cancer in tertiary care cancer centre. We first estimated basic morphological signs: tumor size, tumor grade (by Nottingham Histologic Score), tumor infiltrating lymphocytes (TILs), Lymphovascular invasion, hormonal receptors status, proliferative index, and regional lymph nodes metastasis. The expression of intratumoral neutrophils was studied by CD15 immunohistochemistry which was performed using tissue microarrays. The total number of intratumoral neutrophils, were counted in 5 high-power fields. Results: According to the Nottingham histologic score system, grade I cases were detected in 10 cases (16%), grade II in 34 cases (57%), and grade III in 16 cases (27%). Lymphovascular invasion was determined in 23 cases (38%), and perineural invasion in 14 cases (23%). Number of TILs varied from 0 to 14 (counted in 5 HPF) and averaged 4.2±0.5. Luminal A tumor phenotype was detected in 35 cases (58%), and luminal B HER2-negative in 25 cases (42%). Nineteen (32%) women had metastases in regional lymph nodes (N+). The number of tumor microenvironment neutrophils in luminal HER2-negative breast carcinomas ranged from 1 to 10 (counted in 5 HPF) with an average value of 2.7±0.4. High tumor-associated neutrophils concentration significantly correlated with tumor size (<5mm and >20mm) with p=0.05, high grade (p=0.01), high proliferative index ((r=0.67; p=0.05), TILs (p=0.05), Lymphovascular space invasion (p=0.01)and positive regional lymph nodes metastasis (p=0.001), but not perineural invasion (p=0.1) and also, did not correlate with the expression of estrogen (r=0.18) and progesterone (r=0.14) receptors. Conclusion: Tumor-associated neutrophils strongly predict a worse prognosis in early luminal HER2-negative breast cancer.
Zakurdaev, E. I. , Bagateliya, Z. A. , Titov, K. S. , Elkhouli, E. , Chizhikov, N. P. and Kharina, D. V. (2025). The Role of Tumor-Associated Neutrophils in Early Luminal HER2-Negative Breast Cancer Progression. Asian Pacific Journal of Cancer Prevention, 26(1), 207-213. doi: 10.31557/APJCP.2025.26.1.207
MLA
Zakurdaev, E. I., , Bagateliya, Z. A., , Titov, K. S., , Elkhouli, E. , , Chizhikov, N. P., and Kharina, D. V.. "The Role of Tumor-Associated Neutrophils in Early Luminal HER2-Negative Breast Cancer Progression", Asian Pacific Journal of Cancer Prevention, 26, 1, 2025, 207-213. doi: 10.31557/APJCP.2025.26.1.207
HARVARD
Zakurdaev, E. I., Bagateliya, Z. A., Titov, K. S., Elkhouli, E., Chizhikov, N. P., Kharina, D. V. (2025). 'The Role of Tumor-Associated Neutrophils in Early Luminal HER2-Negative Breast Cancer Progression', Asian Pacific Journal of Cancer Prevention, 26(1), pp. 207-213. doi: 10.31557/APJCP.2025.26.1.207
CHICAGO
E. I. Zakurdaev , Z. A. Bagateliya , K. S. Titov , E. Elkhouli , N. P. Chizhikov and D. V. Kharina, "The Role of Tumor-Associated Neutrophils in Early Luminal HER2-Negative Breast Cancer Progression," Asian Pacific Journal of Cancer Prevention, 26 1 (2025): 207-213, doi: 10.31557/APJCP.2025.26.1.207
VANCOUVER
Zakurdaev, E. I., Bagateliya, Z. A., Titov, K. S., Elkhouli, E., Chizhikov, N. P., Kharina, D. V. The Role of Tumor-Associated Neutrophils in Early Luminal HER2-Negative Breast Cancer Progression. Asian Pacific Journal of Cancer Prevention, 2025; 26(1): 207-213. doi: 10.31557/APJCP.2025.26.1.207