Document Type : Research Articles
Authors
1
Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of b Technology North Bangkok, Bangkok 10800, Thailand.
2
Food and Agro-Industrial Research Center, King Mongkut’s University of Technology North Bangkok, Bangkok 10800,Thailand.
3
Department of Biochemistry, Faculty of Medicine, Princess of Naradhiwas University, Naradhiwas 96000, Thailand.
4
Department of Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam.
5
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
6
AnonBiotec Inc, Patumthani 12120, Thailand.
7
Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.
Abstract
Objective: To evaluate the anti-inflammatory and anticancer effects of Phallus indusiatus extracts, particularly in modulating inflammatory microenvironments and sensitizing lapatinib-induced cytotoxicity in breast cancer cells. Methods: Phallus indusiatus (bamboo mushroom) extracts were prepared using water extraction from fresh and dried specimens. The anti-inflammatory effects were assessed using RAW246.7 cells by measuring NO production, while cytotoxicity and proliferation effects were evaluated in MCF-7 and MCF-10A breast cell lines. To mimic the inflammatory microenvironment of breast cancer, MCF-7 cells were treated with TNFα to induce lapatinib resistance. The effects of the extracts on cell viability, apoptosis, and NF-kappaB signaling were evaluated under inflammatory microenvironment-mimicking conditions. Co-treatment with lapatinib and the extracts was analyzed for synergistic cytotoxic effects and pathway modulation. Results: P. indusiatus extracts demonstrated anti-inflammatory effects by reducing NO production in RAW246.7 cells. The extracts showed no cytotoxicity on MCF-7 and MCF-10A cells, with an observed proliferation increase in MCF-10A but not MCF-7. Under inflammatory microenvironment-mimicking conditions induced by TNFα, the extracts slightly reduced MCF-7 cell viability in a dose-dependent manner. Additionally, the extracts sensitized lapatinib-induced cytotoxicity by increasing apoptotic cell populations. Mechanistically, co-treatment with the extracts attenuated AKT and NF-kappaB activation in a dose-dependent manner. Conclusion: These findings highlight the potential of P. indusiatus extracts as supplementary food for breast cancer patients, particularly in inflammatory microenvironment conditions.
Keywords
Main Subjects
)