Clinical Analysis of Pyroptosis-Related Long Non-Coding RNAs MIAT and Gm15441 in Colorectal Cancer Patients with a History of Ulcerative Colitis

Document Type : Research Articles

Authors

1 Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

2 Alimentary Tract Research Center, Clinical Sciences Research Institute, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.

4 Surgery Department, School of Medicine, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.

5 Clinical Research Development Center (CRDC), Firoozgar Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

6 Occupational Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Pyroptosis can play a significant role in the development of inflammatory bowel diseases such as ulcerative colitis (UC) and colorectal cancer (CRC). The expression pattern of two pyroptosis related-lncRNAs MIAT and Gm15441 was investigated in clinical samples of CRC with and without a history of UC. Methods: In this case-control study, 48 tumor samples from patients with CRC and 48 healthy adjacent tissue samples were studied. A quantitative PCR was completed to analyze the relative expression of lncRNAs GM15441 and MIAT. Quantitative expression levels of the lncRNAs in tumor and healthy tissues were compared. The potential relationship with clinicopathological features and diagnostic values of the lncRNAs were evaluated. Results: The expression levels of both lncRNAs MIAT and Gm15441 were significantly increased in CRC tissues in comparison with healthy tissues (P=0.038 and 0.012, respectively). Also, there was a significant relationship between the expression levels of lncRNA MIAT and lymph node metastasis, but not other clinicopathological characterizations. An AUC of 0.65 for lncRNA MIAT with a sensitivity of 62% and specificity of 54%, with a cut-off value of 2.9 (P= 0.025), and an AUC of 0.66 for lncRNA Gm15441, with a sensitivity of 68% and specificity of 52%, with a cut-off value 1.7 (P= 0.005) were detected. Conclusion: Dysregulated lncRNAs MIAT and Gm15441 in CRC can be more clinically important in cases of cancer related to UC due to their biological function in regulating the inflammation process, providing new ideas about the diagnosis and management of pyroptosis-related diseases.

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Asian Pacific Journal of Cancer Prevention
Volume 26, Issue 2
February 2025
Pages 497-504

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History

  • Receive Date: 30 June 2024
  • Revise Date: 11 December 2024
  • Accept Date: 07 February 2025

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