The Genetic Influence of Programmed Death Ligand-1 (PD-L1) Single Nucleotide Polymorphisms on the Incidence of B-Cell Non-Hodgkin Lymphoma in a Cohort of Egyptians

Document Type : Research Articles

Authors

1 Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.

2 Department of Clinical hematology, Faculty of Medicine, Cairo University, Cairo, Egypt.

Abstract

Objective: This study was conducted to investigate the relationship between rs4143815 (C>G) and rs2890658 (A>C) of the PD-L1 (Programmed Death-Ligand 1) gene and the risk of developing non-Hodgkin’s lymphoma (B-NHL) in a cohort of Egyptian population. Methods: In this case-control study, which included one hundred patients (males and females) diagnosed with B-NHL from Kasr El Aini hematology clinic, their age ranged between 18 and 71 years and 100 age and sex matched healthy controls from outpatient clinic. Three ml venous blood was withdrawn from all subjects and collected in EDTA vacutainer® and kept frozen at -20˚C till time of DNA extraction. DNA samples were extracted from blood samples using GeneJETTM Genomic DNA Purification Kit from Thermo Fisher. Genotyping was determined using Custom TaqMan® SNP Genotyping Assays from Applied biosystems by real-time PCR, and subsequently extensive statistical analysis was performed to investigate the clinical value of these polymorphisms. Patients’ clinical information was obtained from patient’s medical records. Results: The obtained results of the current study demonstrated GG genotype of PD-L1 rs4143815 and the CC genotype of PD-L1 rs2890658 were observed to be more prevalent among NHL patients compared to those reported for the healthy controls (62%, 80% vs 58%, 77%), respectively, however these results revealed no significant association between the studied SNPs of PDL1 gene and risk of NHL (p=0.837, *). Regarding survival analysis, results of 36 and 60 months DFS for  the common genotype (GG) of rs4143815 versus the combined genotype (GC & CC) were (67%, 44.6% vs 63.8%, 63.8%), respectively (p=0.249) as well as results of 36, 60 months DFS for the common genotype (CC) of rs2890658 versus the combined genotype (AA & AC) revealed (61.5%, 46.1% vs 83.3%, 83.3%), respectively which was statistically insignificant (p=0.599), therefore PDL1 rs4143815 & rs2890658 polymorphisms have no significant impact on patients’ DFS. Conclusions: Our findings provided the first evidence that PD-L1 rs4143815 (C>G) and rs2890658 (A>C) are not molecular susceptibility markers for B-NHL in Egyptians, at least in the studied population. 

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Asian Pacific Journal of Cancer Prevention
Volume 26, Issue 2
February 2025
Pages 533-539

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History

  • Receive Date: 13 August 2024
  • Revise Date: 23 December 2024
  • Accept Date: 13 February 2025

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