Serum Level of MVIH, HNF1A-AS1, and NEAT1 Long Noncoding RNAs: Potential Biomarkers for Colorectal Cancer

Mohamadnejad, Mohsen; Firoozi, Mohammad-Reza; Hashemzadeh, Shahriar; Zafari, Venus; Zarredar, Habib; Farzaneh, Rojin; Pedram, Negar; Asvadi Kermani, Touraj

doi:10.31557/APJCP.2025.26.6.1953

Serum Level of MVIH, HNF1A-AS1, and NEAT1 Long Noncoding RNAs: Potential Biomarkers for Colorectal Cancer

Document Type : Research Articles

Authors

¹ Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

² Department of Basic Oncology, Health Institute of Ege University, Izmir, Turkey.

³ Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

10.31557/APJCP.2025.26.6.1953

Abstract

Long noncoding RNAs (lncRNAs) are a significant class of non-coding RNAs that play critical roles in cancer development, progression, and metastasis. This study aimed to investigate the expression of MVIH, HNF1A-AS1, and NEAT1 in the blood samples of patients with colorectal cancer (CRC), both before and three months after surgery, in comparison to a healthy control group. The objective was to assess the potential of these genes as biomarkers for CRC. A total of 75 blood samples were collected from patients diagnosed with colorectal carcinomas (before surgery and at the three-month post-surgery mark), while an additional 75 samples were obtained from a healthy control group. The relative expression levels of the target genes in the serum were measured using quantitative real-time PCR. Our findings demonstrated that the levels of MVIH, HNF1A-AS1, and NEAT1 in the serum were significantly upregulated in CRC subjects compared to the healthy controls. Receiver operating characteristic (ROC) curve analysis revealed that the serum levels of all three genes could effectively distinguish CRC cases from controls with notable accuracy. Furthermore, the serum levels of MVIH, HNF1A-AS1, and HNF1A-AS1 were significantly downregulated three months after surgery. However, no significant correlations were observed between the serum levels of the studied genes and the clinical features of the patients. Collectively, our results suggest that the serum levels of MVIH, HNF1A-AS1, and HNF1A-AS1 have the potential to serve as valuable biomarkers for the diagnosis and prognosis of CRC.

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