The Anticancer Effect of Haematococcus Pluvialis Alga Extract on Breast Cancer Cell Line

Shamskhani, Vahideh; Zarredar, Habib; Razeghi, Jafar; Ansarin, Atefeh; Asadi, Milad; Soleimani, Zahra; Seyedrezazadeh, Ensiyeh

doi:10.31557/APJCP.2025.26.6.2071

The Anticancer Effect of Haematococcus Pluvialis Alga Extract on Breast Cancer Cell Line

Document Type : Research Articles

Authors

¹ Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

² Tuberculosis and Lung Diseases Research Canter, Tabriz University of Medical Sciences, Tabriz, Iran.

³ Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey.

⁴ Rahat Breathe and Sleep Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

10.31557/APJCP.2025.26.6.2071

Abstract

Objective: The aim of this study was to investigate the anticancer effects of H. pluvialis extract on breast cancer. Methods: We evaluated the cytotoxicity, suppression of migration, and apoptosis ability of H. pluvialis alga extract MDA-MB-468 by MTT assay, scratch test, and flow cytometry, respectively. The expression patterns of MMP1 and MMP9, Bax and Bcl2, Caspase3, Caspase6, and Caspase9 were analyzed by qRT-PCR. Results: Based on the findings of this study, the survival and proliferation capacity of MDA-MB-468 cells treated with the extract was reduced. Flow cytometry results showed that treatment with H. Pluvialis extracts induced apoptosis. Scratch test results showed that H. Pluvialis extract could inhibit cell migration. The expression patterns of the studied genes were changed by treatment with the extract. Expression of Bcl2, MMP1, and MMP9 genes decreased, and expression of Bax, Caspase3, Caspase8, Caspase9 genes increased. Conclusion: The findings of this research reveal that H. pluvialis algae extract shows a potential anti-cancer compound.

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