The Significant Role of RUNX2 Expression in Women with Metastatic Breast Cancer

Rezaei, Mitra; Mansouri, Neda; Sohbatzadeh, Zihab; Ghadyani, Rezvaneh; Soleimani, Shahrzad; Motallebi, Marzieh; Parsa Zad, Ehsan; Torkaman, Maryam; Kolahi, Ali Asghar; Movafagh, Abolfazl

doi:10.31557/APJCP.2025.26.7.2635

The Significant Role of RUNX2 Expression in Women with Metastatic Breast Cancer

Document Type : Research Articles

Authors

¹ Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

² Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca). IBSAL and CIBERONC, Salamanca, Spain.

³ Department of Physics, University of Sistan and Baluchestan, Zahedan, Iran.

⁴ Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

⁵ Department of Molecular Genetics, Institute of Basic Science, Shahrekord Islamic Azad University, Iran.

⁶ Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

⁷ Department of Bioscience and Biotechnology, Malek Ashtar University, Tehran, Iran.

⁸ Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

10.31557/APJCP.2025.26.7.2635

Abstract

Objective: Breast cancer (BC) is one of the most common malignancies with second ranks among the causes of tumor-associated mortality in females. Since the overexpression of RUNX2 gene were reported in the development of different malignancies. The present investigation was aimed to find its correlation with prognostic factors viz, tumor grade and stage in the breast cancer patients. Methods: This study, examined the expression level changes of RUNX2 genes with the Real-time PCR and compared the results with various databases and previous research results, to understand the existence of differences between metastatic breast cancer patients’ samples and non-metastatic BC samples of patients. Results: The prepared samples were divided into two target and control groups and different analyses after QPCR reaction were accomplished to understand the level of changes and significance. Conclusion: Results of this study suggest that an elevated RUNX2 gene expression level could be a valuable independent predictor of tumor metastasis and survival prediction in invasive breast cancer patients. However, to reach more reliable results and better conclusions more samples, studies, and techniques are needed.

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