Biosynthesis of Copper Nanoparticles Using Passiflora Flower Extract and Study Their Antimicrobial, Antioxidant And Anticancer Activity

Sahib, Ons H; Mohammed, Lubna Z; Mahdi, Montadher Ali

doi:10.31557/APJCP.2025.26.8.2953

Biosynthesis of Copper Nanoparticles Using Passiflora Flower Extract and Study Their Antimicrobial, Antioxidant And Anticancer Activity

Document Type : Research Articles

Authors

¹ College of Science, Mustansiriyah University, Baghdad, Iraq.

² College of Science for Women, University of Baghdad, Baghdad, Iraq.

³ National Center for Cancer Research, University of Baghdad, Baghdad, Iraq.

10.31557/APJCP.2025.26.8.2953

Abstract

Objective: Copper nanoparticles (CuNPs) have received interest due to their antibacterial, antioxidant, and anticancer effects. However, current synthesis methods frequently use harmful substances. Green synthesis with plant extracts provides a safer, more environmentally friendly option. This study looks at the production of CuNPs with Passiflora flower extract and assesses their biological activity. Methods: Passiflora extract was used as a reducing and stabilizing agent in the synthesis of CuNPs. UV-Vis spectroscopy, FTIR, SEM, XRD, and AFM were used to characterize the samples. The biological activities were evaluated using DPPH antioxidant assays, antimicrobial tests against bacterial and fungal strains, and MTT assays on MCF7 cancer cells and HFF normal cells. Results: The CuNPs produced had a spherical form with an average size of 10-20 nm. Antioxidant activity was high, with an IC50 of 98.94 µg/ml. CuNPs showed broad-spectrum antibacterial activity, especially against Staphylococcus aureus and Candida albicans. The cytotoxicity assay revealed preferential toxicity against MCF7 breast cancer cells (IC50 = 47.01 µg/ml) while preserving normal HFF cells (IC50 = 128.35 µg/ml). These findings are consistent with prior research, confirming the potential biomedical applications of CuNPs. Conclusion: Green-synthesized CuNPs have substantial antioxidant, antibacterial, and anticancer properties. Their specific toxicity to cancer cells demonstrates their potential as a Nano medicine option. Further research into in vivo efficacy and long-term safety is recommended.

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