Evaluation of Genetic Polymorphisms of HOTTIP (rs5883064, rs1859168, rs2067087) in Patients with Non-Hodgkin Lymphoma and Comparison with Healthy Subjects

Sadraei, Masoomeh; Hashemi, Seyed Mehdi; Mozaffari, Hossein; Taheri, Mohsen; Mahdizadeh, Reza; Bahari, Gholamreza

doi:10.31557/APJCP.2025.26.10.3857

Evaluation of Genetic Polymorphisms of HOTTIP (rs5883064, rs1859168, rs2067087) in Patients with Non-Hodgkin Lymphoma and Comparison with Healthy Subjects

Document Type : Research Articles

Authors

¹ Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

² Student Research Committee, Zahedan University of Medical Sciences. Zahedan, Iran.

³ Clinical Immunology Research Center, Department of Internal Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

⁴ Genetics of Non- Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.

⁵ Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.

10.31557/APJCP.2025.26.10.3857

Abstract

Background: Non-Hodgkin Lymphoma (NHL) is a group of blood cancers in which lymphoid cells develop abnormal growth due to genetic changes or other factors without Reed-Sternberg cells. Given the proven role of HOTTIP gene polymorphisms in the prognosis, diagnosis, and treatment of different kinds of carcinomas, this study investigated the association between rs5883064, rs1859168, and rs2067087 polymorphisms of the gene with NHL susceptibility in a population of Zahedan. Materials and Methods: A total of 190 NHL cases (122 males and 68 females), aged 44.45±15.29 years, and 190 healthy individuals (107 males and 83 females, 43.13±12.15 years) were collected as study subjects. The gene polymorphisms were examined by PCR RFLP and ARMS methods. Results: Our results showed that the rs5883064 Del>Ins polymorphism of the HOTTIP gene has a protective role and significantly reduces the risk of lymphoma. The 1859168 C>A variant in the co-dominant model is a risk criterion for non-Hodgkin lymphoma and significantly increases this disease’s risk. The HOTTIP rs2067087 G>C variant had a protective role and significantly reduced the risk of lymphoma. Conclusion: Our results showed that the association between HOTTIP gene polymorphisms and NHL incidence has a significant pattern. More studies in larger populations and various ethnicities are recommended to confirm our findings.

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