Tempeh Extract and HSC-3 Cell Migration: An In Vitro Study

Amtha, Rahmi; Tjhie, Shirley; Hartanto, Firstine Kelsi; Hayuningtyas, Ria Aryani; Gunardi, Indrayadi; Hussaini, Haizal

doi:10.31557/APJCP.2025.26.12.4465

Tempeh Extract and HSC-3 Cell Migration: An In Vitro Study

Document Type : Research Articles

Authors

¹ Department of Oral Medicine, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.

² Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.

³ Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.

⁴ Department of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, New Zealand.

10.31557/APJCP.2025.26.12.4465

Abstract

Objective: To compare the migration capacity of HSC-3 cells following treatment with various concentrations of tempeh extract. Methods: This laboratory-based study using HSC-3 cell lines, which were treated with tempeh extract (SNI-3144:2015) dissolved in either 70% ethanol or water at concentrations of 25, 50, 100, 200, and 400 μg/mL. Cell migration was assessed using a scratch assay at 6 and 24 hours, conducted in triplicate. Data were analyzed using ANOVA and Kruskal–Wallis tests. Result: At the 6-hour observation point, no significant differences in migration were observed between groups for either solvent (p>0.05). However, after 24 hours, the 50 μg/mL group (6.88±11.92) demonstrated the largest scratch area compared with other concentrations (p<0.033). Conclusion: Tempeh extract showed no inhibitory effect on HSC-3 cell migration when compared to doxorubicin, particularly when water was used as the solvent. Further research is needed with higher concentrations of tempeh extract to evaluate its potential inhibitory effects on HSC-3 cell migration.

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