Mitogen-Activated Protein Kinase-3 (MAPK3) Is the Main Target of Microsecond Pulsed Electric Field in Human Medulloblastoma

Document Type : Research Articles

Authors

1 Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Skin Research Center, Shahid Beheshti University of medical sciences, Tehran, Iran.

4 Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

5 Iranian Cancer Control Center (MACSA), Tehran, Iran.

6 Proteomics Research Center, System Biology Institute, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

7 Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran

8 Department of Community Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

9 Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

10 Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Pulsed electric fields (PEFs) are applied to facilitate the transfer of various types of molecules, such as pharmaceuticals, into living cells. In the present study effect of microsecond PEF on human medulloblastoma cell line (D283Med cells) is investigated to find the crucial targeted genes. Methods: Gene expression profiles of the human medulloblastoma (D283) cell line in response to microsecond PEF versus control are extracted from the Gene Expression Omnibus (GEO) database. The samples are analyzed via the GEO2R program to find the significant differentially expressed genes (DEGs). The significant DEGs were assessed via protein-protein interaction (PPI) network using Cytoscape software and its applications. Results: Among 410 significant DEGs, 23 hubs and bottlenecks were determined. A total number of seven hub-bottlenecks including MAPK3, KIT, APP, RUNX2, CXCR4, LMNA, and BMP4 were pointed out as the crucial targets of microsecond PEF. MAPK3 and KIT were down-regulated while, APP, RUNX2, CXCR4, LMNA, and BMP4 were up-regulated. Conclusion: It can be concluded that, down-regulation of MAPK3 and KIT and up-regulation of BMP4 which are the consequence of microsecond PEF treatment inhibit medulloblastoma. The results exhibited the significant role of MAPK3 in response to microsecond PEF treatment.

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Asian Pacific Journal of Cancer Prevention
Volume 27, Issue 2
February 2026
Pages 463-468

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History

  • Receive Date: 09 March 2025
  • Revise Date: 07 September 2025
  • Accept Date: 26 January 2026

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