Document Type : Research Articles
Authors
1
Department of Radiological Technology, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand.
2
Chulabhorn International College of Medicine (CICM), Thammasat University, Pathum Thani, 12120, Thailand.
3
Research Group in Multidimensional Health and Disease (MHD), Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand.
4
Thammasat Research Unit in Opisthorchiasis, Cholangiocarcinoma, and Neglected parasitic Diseases (TRU-OCN), Thammasat University, Pathumthani, 12120, Thailand.
5
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
6
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
7
Medical Oncology Unit, Udonthani Cancer Hospital, Udon Thani, 41330, Thailand.
8
Faculty of Science, Udon Thani Rajabhat University, Udon Thani, 41000, Thailand.
Abstract
Objective: To investigate plasma miR-1294 levels in cholangiocarcinoma (CCA) patients compared with healthy individuals and Opisthorchis viverrini-infected subjects. Methods: To assess its potential as a plasma-based diagnostic biomarker, plasma samples were collected and total RNA was extracted from 15 healthy controls (HC), 12 O. viverrini (OV)-infected individuals, and 33 patients with CCA. miR-1294 expression was quantified using RT-qPCR (MiRXES). Results: Relative expression analysis demonstrated a significant upregulation of plasma miR-1294 in patients with CCA compared with both HC and OV groups (P < 0.0001). Receiver operating characteristic (ROC) curve analysis showed that miR-1294 discriminated CCA from non-CCA subjects, yielding an area under the curve (AUC) of 0.8676, with a specificity of 96.30% and a sensitivity of 63.64%. Conclusion: The upregulation of plasma miR-1294 was investigated in this study. Despite these promising findings, the moderate sensitivity suggests that miR-1294 alone may not be sufficient as a standalone diagnostic marker. Nevertheless, its high specificity and non-invasive detectability highlight its potential utility as a complementary biomarker, particularly in combination with other diagnostic indicators for improved detection of CCA.
Keywords
Main Subjects
)