Background: Micro RNAs (miRNAs) are small, noncoding RNA molecules. They can function as either oncogenes or tumor suppressor genes. Single nucleotide polymorphisms (SNP) present in the pre-miRNA region could affect the processing of miRNA and thus alter mature miRNA expression. The studies done so far had shown conflicting results regarding association of two common polymorphisms i.e.hsa-miR-146 rs2910164 and hsa-miR-196a2 rs11614913 with breast cancer. OBJECTIVE: In the study, we examined the hsa-miR-146 rs2910164 and hsa-miR-196a2 rs11614913 SNP association with breast cancer patients in north Indian women. Materials and Methods: This study included 100 breast cancer patients and 100 controls and was done over a period of two years. Genotypes of the hsa-miR-146 (rs2910164 G>C) and hsa-miR-196a2 (rs11614913 C>T) were identified by polymerase chain reaction – restriction length polymorphism (PCR-RFLP) technique in peripheral blood DNA samples. Statistical analysis: We assessed the strength of association of miRNA polymorphisms with breast cancer using Odds ratio (OR) along with 95% confidence intervals. Results: Heterozygous genotypes of hsa-miR-196a2 rs11614913 and combined hsa-miR-146 rs2910164 & hsa-miR-196a2 polymorphism were associated with significantly increased risk of breast cancer (OR-1.7, 95% CI–1.00-3.18) and (OR-1.9, 95% CI-0.85-4.46) respectively. Conclusion: Our study suggests that rs2910164 GC and rs11614913 CT genotypes may contribute to breast cancer susceptibility in north Indian women.
Bodal, V. K., Sangwan, S., Bal, M., Kaur, M., Sharma, S., & Kaur, B. (2017). Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women. Asian Pacific Journal of Cancer Prevention, 18(9), 2345-2348. doi: 10.22034/APJCP.2017.18.9.2345
MLA
Vijay Kumar Bodal; Shruti Sangwan; Manjit Bal; Mohanvir Kaur; Sidarth Sharma; Bhavleen Kaur. "Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women". Asian Pacific Journal of Cancer Prevention, 18, 9, 2017, 2345-2348. doi: 10.22034/APJCP.2017.18.9.2345
HARVARD
Bodal, V. K., Sangwan, S., Bal, M., Kaur, M., Sharma, S., Kaur, B. (2017). 'Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women', Asian Pacific Journal of Cancer Prevention, 18(9), pp. 2345-2348. doi: 10.22034/APJCP.2017.18.9.2345
VANCOUVER
Bodal, V. K., Sangwan, S., Bal, M., Kaur, M., Sharma, S., Kaur, B. Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women. Asian Pacific Journal of Cancer Prevention, 2017; 18(9): 2345-2348. doi: 10.22034/APJCP.2017.18.9.2345
)