Background: In order to find cytogenetic and molecular metastasis biomarkers detectable in peripheral blood the spontaneous genomic instability expressed as micronuclei and Bmi-1 expression in peripheral blood of breast cancer (BC) patients were studied in different stages of the disease compared with unaffected first-degree relatives (FDRs) and normal control. Methods: The Cytokinesis Block Micronuclei Cytome (CBMN cyt) and nested real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) assays, were respectively used to measure genomic instability and Bmi-1 gene expression in 160 Iranian individuals comprised of BC patients in different stages of the disease, unaffected FDRs and normal control groups. Result: The frequency of micronuclei and Bmi-1 expression were dramatically higher in distant metastasis compared with non-metastatic BC. In spite of micronucleus frequency with no association with lymph node (LN) involvement and hormone receptor status, the Bmi-1 expression level was higher in LN positive and triple negative patients. Conclusion: Our results indicate that increased genomic instability expressed as micronuclei and higher Bmi-1 expression in peripheral blood are associated with metastasis in breast cancer. Therefore implementation of micronucleus assay and Bmi-1 expression analysis in blood as possible cytogenetic and molecular biomarkers in clinical level may potentially enhance the quality of management of patients with breast cancer.
Salimi, M. and Eskandari, E. (2018). Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients. Asian Pacific Journal of Cancer Prevention, 19(10), 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
MLA
Salimi, M. , and Eskandari, E. . "Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients", Asian Pacific Journal of Cancer Prevention, 19, 10, 2018, 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
HARVARD
Salimi, M., Eskandari, E. (2018). 'Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients', Asian Pacific Journal of Cancer Prevention, 19(10), pp. 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
CHICAGO
M. Salimi and E. Eskandari, "Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients," Asian Pacific Journal of Cancer Prevention, 19 10 (2018): 2723-2730, doi: 10.22034/APJCP.2018.19.10.2723
VANCOUVER
Salimi, M., Eskandari, E. Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients. Asian Pacific Journal of Cancer Prevention, 2018; 19(10): 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
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