Background: In order to find cytogenetic and molecular metastasis biomarkers detectable in peripheral blood the spontaneous genomic instability expressed as micronuclei and Bmi-1 expression in peripheral blood of breast cancer (BC) patients were studied in different stages of the disease compared with unaffected first-degree relatives (FDRs) and normal control. Methods: The Cytokinesis Block Micronuclei Cytome (CBMN cyt) and nested real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) assays, were respectively used to measure genomic instability and Bmi-1 gene expression in 160 Iranian individuals comprised of BC patients in different stages of the disease, unaffected FDRs and normal control groups. Result: The frequency of micronuclei and Bmi-1 expression were dramatically higher in distant metastasis compared with non-metastatic BC. In spite of micronucleus frequency with no association with lymph node (LN) involvement and hormone receptor status, the Bmi-1 expression level was higher in LN positive and triple negative patients. Conclusion: Our results indicate that increased genomic instability expressed as micronuclei and higher Bmi-1 expression in peripheral blood are associated with metastasis in breast cancer. Therefore implementation of micronucleus assay and Bmi-1 expression analysis in blood as possible cytogenetic and molecular biomarkers in clinical level may potentially enhance the quality of management of patients with breast cancer.
Salimi, M., & Eskandari, E. (2018). Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients. Asian Pacific Journal of Cancer Prevention, 19(10), 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
MLA
Mahdieh Salimi; Elaheh Eskandari. "Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients". Asian Pacific Journal of Cancer Prevention, 19, 10, 2018, 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
HARVARD
Salimi, M., Eskandari, E. (2018). 'Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients', Asian Pacific Journal of Cancer Prevention, 19(10), pp. 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
VANCOUVER
Salimi, M., Eskandari, E. Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients. Asian Pacific Journal of Cancer Prevention, 2018; 19(10): 2723-2730. doi: 10.22034/APJCP.2018.19.10.2723
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