Campylobacter Concisus and Its Effect on the Expression of CDX1 and COX2

Document Type : Research Articles

Authors

1 Department of Microbiology of Pathobiology, School of Public Health, Tehran University of Medical Sciences, International Campus (TUMS-IC), Tehran, Iran.

2 Microbiology and Gut Biology Group, University of Dundee, Ninewells Hospital Medical School, Dundee, UK.

3 Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

4 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Barrett’s oesophagus (BO) is a pre-malignant condition in which normal squamous epithelium of the
lower oesophagus and gastresophageal junction is replaced by columnar cells and progress to oesophageal adenocarcinoma.
The increase burden of oesophagus cancer morbidity and mortality worldwide make study of factors involved in the
pathogenesis of BO essential. However, most of studies that examine the environmental risk factors associated with
increased incidence and prevalence of BO have largely ignored the potential role of bacteria in disease aetiology.
Aims: This study examined the role of Campylobacter concisus isolated from Barrett’s and adenocarcinoma
patient samples as one of possible environmental factors in the progression of Barrett’s oesophagus to oesophagus
adenocarcinoma. Methods: We focused on the effect of C. concisus on the expression caudal type homeobox 1 gene
(CDX1) and cyclooxygenase-2 (COX-2) in three BO cell lines using quantitative real-time PCR. In addition, the
attachment and invasion characteristics of C. concisus were also tested. Results: Results showed that C. concisus had a
strong attachment to the cell lines and induce the expression of CDX1 in Barrett’s cell lines in a time-dependent manner.
Conclusion: Findings indicate that C. concisus could be as a new challenge in the progression of BO to adenocarcinoma.

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References

 
Asian Pacific Journal of Cancer Prevention
Volume 19, Issue 11
November 2018
Pages 3211-3216

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History

  • Receive Date: 06 May 2018
  • Revise Date: 06 August 2018
  • Accept Date: 05 October 2018

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