Document Type : Research Articles
Authors
1
Department of Microbiology of Pathobiology, School of Public Health, Tehran University of Medical Sciences, International Campus (TUMS-IC), Tehran, Iran.
2
Microbiology and Gut Biology Group, University of Dundee, Ninewells Hospital Medical School, Dundee, UK.
3
Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
4
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
Background: Barrett’s oesophagus (BO) is a pre-malignant condition in which normal squamous epithelium of the
lower oesophagus and gastresophageal junction is replaced by columnar cells and progress to oesophageal adenocarcinoma.
The increase burden of oesophagus cancer morbidity and mortality worldwide make study of factors involved in the
pathogenesis of BO essential. However, most of studies that examine the environmental risk factors associated with
increased incidence and prevalence of BO have largely ignored the potential role of bacteria in disease aetiology.
Aims: This study examined the role of Campylobacter concisus isolated from Barrett’s and adenocarcinoma
patient samples as one of possible environmental factors in the progression of Barrett’s oesophagus to oesophagus
adenocarcinoma. Methods: We focused on the effect of C. concisus on the expression caudal type homeobox 1 gene
(CDX1) and cyclooxygenase-2 (COX-2) in three BO cell lines using quantitative real-time PCR. In addition, the
attachment and invasion characteristics of C. concisus were also tested. Results: Results showed that C. concisus had a
strong attachment to the cell lines and induce the expression of CDX1 in Barrett’s cell lines in a time-dependent manner.
Conclusion: Findings indicate that C. concisus could be as a new challenge in the progression of BO to adenocarcinoma.
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