Preventive Effects of Fermented Brown Rice and Rice Bran on Spontaneous Lymphomagenesis in AKR/NSlc Female Mice

Document Type : Research Articles


1 Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Nagoya, Japan.

2 Division of Diagnostic Pathology, Gifu Municipal Hospital, 7-1 Kashima-cho, 7-1, Gifu, Japan.

3 Emeritus Professor of Gifu University, 1-1 Yanagido, Gifu, Japan.


Fermented brown rice and rice bran with Aspergillus oryzae (FBRA) is known to possess potentials to prevent
chemical carcinogenesis in multiple organs of rodents. In the present study, possible chemopreventive effect of FBRA
against spontaneous occurrence of lymphomas was examined using female AKR/NSlc mice. Four-week-old female AKR/
NSlc mice were divided into three groups, and fed diets containing FBRA for 26 weeks at a dose level 0% (Group 1), 5%
(Group 2) or 10% (Group 3). At the termination of experiment, the incidence of thymic malignant lymphoma of Group
3 was significantly lower than of Group 1 (p < 0.05). The average number of apoptotic cells of the thymic lymphoma of
Group 3 was significantly larger than that of Group 1 (p < 0.05). In addition, the incidences of malignant lymphoma
arising from body surface and abdominal lymph nodes, and the frequencies of lymphoma cell invasion to liver, kidney,
spleen, and ovary of Group 3 were relatively lower than those of Group 1. These results indicate that FBRA inhibits
spontaneous development of the lymphoma in female AKR/NSc mice and the inhibition of lymphomagenesis may
relate to the induction of apoptosis by exposure of FBRA, suggesting that FBRA could be a protective agent against
development of human lymphoma.




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