CD56 and CD11b Positivity with Low Smac/DIABLO Expression as Predictors of Chemoresistance in Acute Myeloid Leukaemia: Flow Cytometric Analysis

Ibrahim, Abeer; Zahran, Asmaa M; Aly, Sanaa Shaker; Refaat, Ahmed; Hassan, Mohammed H

doi:10.31557/APJCP.2018.19.11.3187

CD56 and CD11b Positivity with Low Smac/DIABLO Expression as Predictors of Chemoresistance in Acute Myeloid Leukaemia: Flow Cytometric Analysis

Document Type : Research Articles

Authors

¹ Department of Medical Oncology and Haematological Malignancies, South Egypt Cancer Institute, Assiut University, Egypt.

² Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Egypt.

³ Department of Clinical Pathology, Faculty of Medicine, South Valley University, Qena, Egypt.

⁴ Department of Medical Biochemistry-Faculty of Medicine- South Valley University, Qena, Egypt.

10.31557/APJCP.2018.19.11.3187

Abstract

Background: Resistance to chemotherapy is a major obstacle to curing acute myeloid leukaemia (AML), and
several antigens are claimed to play primary roles in this resistance. Purpose: The aim of this study was to evaluate
the roles of CD56, CD11b and Smac/DIABLO gene expression levels as prognostic markers of the clinical outcome,
response to chemotherapy and survival of AML patients. Materials and Methods: A cross-sectional observational
study was conducted on 60 naïve-AML patients who received induction therapy with mitoxantrone and cytarabine
combined with a high dose of cytarabine. The CD56,CD11b and Smac/DIABLO expression levels were assessed using
flow cytometry at diagnosis and were analysed for correlation with the possible associated risk factors, response to
chemotherapy, and median duration of disease-free survival (DFS) and overall survival (OS). Results: The overall results
revealed that AML patients who exhibited positive expression for CD56 and CD11b had short median durations of DFS
and OS.(P = 0.019, 0.006, 0.029 and 0.024, respectively). Additionally, low Smac/DIABLO expression had a negative
impact on treatment outcome in terms of CR rate (p=0.012) and reduced DFS (p=0.000) and OS(p=0.000) values.
Conclusions: CD56 and CD11b positivity and low Smac/DIABLO expression are important predictive factors for
the occurrence of chemoresistance, in addition to other risk factors, among AML patients.

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