Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells

Document Type : Research Articles

Authors

1 Postgraduate Program of Pharmaceutical Sciences of Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.

2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.

3 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Abstract

Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single
compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells,
selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flow
cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine did
not significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of
cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic
proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin.
Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells
through modulation of the cell cycle and affecting the expression of caspases 3 and 9.

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References

 
Asian Pacific Journal of Cancer Prevention
Volume 19, Issue 11
November 2018
Pages 3065-3069

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History

  • Receive Date: 30 October 2017
  • Revise Date: 03 July 2018
  • Accept Date: 22 October 2018

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