Relapse and Survival in Bladder Cancer Patients Undergoing microRNA-129 and microRNA-145 Assays

ELfieky, Mostafa M M; Abd El Rahman, Mohamed; Fayed, Aysam M; Haleem Al-Qaim, Zahraa; Khalid Aldhalmi, Ahmed; AE Badr, Eman; Abdel Aziz, Amal; Ibrahim, Gehan M A

doi:10.31557/APJCP.2024.25.6.2113

Relapse and Survival in Bladder Cancer Patients Undergoing microRNA-129 and microRNA-145 Assays

Document Type : Research Articles

Authors

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.

² College of Pharmacy, Al-Mustaqbal University, Babylon, 51001, Iraq.

³ Clinical Pharmacy Department, Badr University Hospital, Faculty of Medicine, Helwan University, Egypt.

⁴ Medical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon,51001, Iraq.

⁵ Department of Anesthesia Techniques, Al-Mustaqbal University College, Iraq.

⁶ Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia University, Egypt.

10.31557/APJCP.2024.25.6.2113

Abstract

Objective: The lack of indicators to measure tumor’s invasive biological behavior is an important issue. The aim of this study was to examine the effect of miRNAs 129 and 145 on tumor progression as well as patient survival. Method: Seventy five breast cancer (BC) patients and 75 controls were included in this research. Two miRNA expressions were estimated using real-time PCR. Biomarkers for BC detection was tested using ROC curves and AUC. Result: miR-129 and miR-145 expressions were significant. miR-129 and miR-145 classifiers (AUC = 0.943 and 0.748, respectively) help diagnose BC. Unlike miR-145, miR-129 did not affect the Kaplan–Meier survival curve analysis for progression-free survival at the end of the trial. The development of transitional cell carcinoma disease was found to have a strong correlation with miR-145 in both univariate and multivariate Cox regression analyses. Additionally, infiltrating + invasive urothelial carcinoma was also found to be correlated with miR-145. Conversely, elevated miR-129 expression in BC patients did not lead to an increase in cancer-specific recurrence or mortality, as observed in both univariate and multivariate Cox regression studies. Conclusion: The miRNA signature can help detect survival-associated miRNAs and develop BC miRNA therapeutics.

Keywords