Document Type : Research Articles
Authors
1
Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
2
Department of Microbiology, ZA.C., Islamic Azad University, Zanjan, Iran.
3
Department of Biology, Harold Washington College, City College of Chicago, Chicago, IL, USA.
4
School of Pharmacy, Sonderegger Research Center, University of Wisconsin, Madison, 777 Highland Avenue, Madison, WI 53705-2222, United States.
5
Assistant Professor of Pediatrics, Department of Pediatrics, Ali Asghar Children’s Hospital, Iran University of Medical Sciences, Tehran, Iran.
6
Chemistry Department, College of Science, University of Tehran, Tehran, Iran.
7
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
8
Department of Pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.
9
Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza, Iran.
Abstract
Background: This study investigates the green synthesis of selenium nanoparticles (SeNPs) and their encapsulation in liposomes as a novel drug delivery system to enhance the antibacterial and anticancer properties of SeNPs. Liposomes are well-known for their ability to improve the biological activity of encapsulated drugs, making them a promising candidate for targeted therapies, particularly in oral cancer treatment. Methods: Biosynthesised SeNPs were incorporated into liposomes via the thin-film hydration technique. Particle size and zeta potential were quantified by dynamic light scattering (DLS), whereas encapsulation efficiency (EE) was determined spectrophotometrically (UV–Vis). Results: The physicochemical properties of the liposome-loaded SeNPs were characterized, revealing an average size of 270 nm, spherical morphology, and an encapsulation efficiency of 50.5%. The release profile of SeNPs from the liposomes demonstrated a controlled release of 61% over 64 hours, while free SeNPs released 100% of their content during the same period. The antibacterial and anti-biofilm activities of both free and liposome-loaded SeNPs were tested against standard pathogenic bacterial strains, with the liposome formulation showing enhanced efficacy. The cytotoxicity assay revealed that liposome-loaded SeNPs exhibited significantly higher cytotoxic effects on oral cells compared to free SeNPs, indicating improved therapeutic potential. Conclusion: The study demonstrates that liposome-loaded SeNPs are an effective and biocompatible drug delivery system with notable antibacterial, anti-biofilm, and anticancer properties, making them a promising candidate for targeted drug delivery in oral cancer therapy.
Keywords
Main Subjects
)